Record Details

Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae.

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae.
 
Creator Bourbouloux, Andrée
Shahi, Puja
Chakladar, A
Delrot, Serge
Bachhawat, Anand K
 
Subject QD Chemistry
 
Description A high affinity glutathione transporter has been identified, cloned, and characterized from the yeast Saccharomyces cerevisiae. This transporter, Hgt1p, represents the first high affinity glutathione transporter to be described from any system so far. The strategy for the identification involved investigating candidate glutathione transporters from the yeast genome sequence project followed by genetic and physiological investigations. This approach revealed HGT1 (open reading frame YJL212c) as encoding a high affinity glutathione transporter. Yeast strains deleted in HGT1 did not show any detectable plasma membrane glutathione transport, and hgt1Delta disruptants were non-viable in a glutathione biosynthetic mutant (gsh1Delta) background. The glutathione repressible transport activity observed in wild type cells was also absent in the hgt1Delta strains. The transporter was cloned and kinetic studies indicated that Hgt1p had a high affinity for glutathione (K(m) = 54 micrometer)) and was not sensitive to competition by amino acids, dipeptides, or other tripeptides. Significant inhibition was observed, however, with oxidized glutathione and glutathione conjugates. The transporter reveals a novel class of transporters that has homologues in other yeasts and plants but with no apparent homologues in either Escherichia coli or in higher eukaryotes other than plants.
 
Publisher ASBMB
 
Date 2000-05-05
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/832/1/bachhawat2000.pdf
Bourbouloux, Andrée and Shahi, Puja and Chakladar, A and Delrot, Serge and Bachhawat, Anand K (2000) Hgt1p, a high affinity glutathione transporter from the yeast Saccharomyces cerevisiae. The Journal of biological chemistry, 275 (18). pp. 13259-65. ISSN 0021-9258
 
Relation http://www.jbc.org/content/275/18/13259.long
http://crdd.osdd.net/open/832/