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Cloning, expression, and sequence analysis of the gene encoding the alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic Bacillus sp. Strain NG-27.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Cloning, expression, and sequence analysis of the gene encoding the alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic Bacillus sp. Strain NG-27.
 
Creator Gupta, N
Reddy, V S
Maiti, S
Ghosh, A
 
Subject QR Microbiology
 
Description Alkalophilic Bacillus sp. strain NG-27 produces a 42-kDa endoxylanase active at 70 degrees C and at a pH of 8.4. The gene for this endoxylanase was cloned and sequenced. The gene contained one open reading frame of 1,215 bases. An active site characteristic of the family 10 beta-glycanases was recognized between amino acids 303 and 313, with the active glutamate at position 310. Though highly thermostable, the enzyme contains no cysteine residue.
 
Publisher ASM
 
Date 2000-06
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/834/1/ghosh2000.pdf
Gupta, N and Reddy, V S and Maiti, S and Ghosh, A (2000) Cloning, expression, and sequence analysis of the gene encoding the alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic Bacillus sp. Strain NG-27. Applied and environmental microbiology, 66 (6). pp. 2631-5. ISSN 0099-2240
 
Relation http://aem.asm.org/content/66/6/2631.full.pdf+html
http://crdd.osdd.net/open/834/