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Identification of four genes involved in suppression of the pre-mRNA splicing defect in the sng1-1/rhp6- mutant of fission yeast

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Identification of four genes involved in suppression of the pre-mRNA splicing defect in the sng1-1/rhp6- mutant of fission yeast
 
Creator Naresh, Alpana
Singh, Jagmohan
 
Subject QH426 Genetics
 
Description Apart from the global regulators of silencing in the fission yeast Schizosaccharomyces pombe, namely swi6, clr1,
clr2, clr3, clr4 and rik1, the DNA repair gene rhp6 plays a unique role in mating-type silencing. Recently, we showed
that sng1-1, a mutation in the 5' splice junction of the second intron of the rhp6 gene, leads to derepression of both the
silent loci mat2 and mat3 in switching background. To address the mechanism of rhp6 in silencing, we have isolated
several extragenic suppressors of the sng1-1/rhp6– mutation. These suppressors fall into four complementation groups
and are referred to as suppressor of rhp6: sur1, sur2, sur3 and sur4. Interestingly, reverse transcriptase polymerase
chain reaction analysis of the rhp6 transcript shows that in contrast to about > 50% level of unspliced rhp6 pre-mRNA
in the sng1-1/rhp6– mutant, there is a restoration of normal splicing to varying degrees in the suppressors. The sur2 gene belongs to the AAA-ATPase family of proteins, with maximum homology to the SIN1-associated protein SAP1
of Saccharomyces cerevisiae. We propose that sur2, along with sur1, sur3 and sur4, may play an as yet uncharacterized
role in pre-mRNA splicing.
 
Publisher IAS
 
Date 2000
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/841/1/jagmohan2000.pdf
Naresh, Alpana and Singh, Jagmohan (2000) Identification of four genes involved in suppression of the pre-mRNA splicing defect in the sng1-1/rhp6- mutant of fission yeast. Journal of Genetics, 79. pp. 83-90.
 
Relation http://www.ias.ac.in/jgenet/Vol79No3/JG473.PDF
http://crdd.osdd.net/open/841/