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An evaluation of models for the effect of plasmid copy number on bacterial growth rate.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title An evaluation of models for the effect of plasmid copy number on bacterial growth rate.
 
Creator Patnaik, P R
 
Subject QR Microbiology
 
Description The rates of growth of recombinant bacteria depend on their plasmid content. This is modelled by expressing
the specific growth rate in terms of the number of copies of the plasmid per cell. Three models in common use
have been tested with different Escherichia coli strains and one strain of Bacillus stearothermophilus containing
different plasmids. While no particular model was decisively better than others for all data, that of Bentley & Quiroga (Biotechnol. Bioeng. 1993, 42: 222–234) was the best for specific growth rates which vary inversely with
the plasmid copy number, and a modified form of the model of Satyagal & Agarwal (Biotechnol. Bioeng. 1989,
33: 1135–1144) was the best for growth rates which increase with the copy number. The differences appear to be
linked to the plasmid replication mechanisms. Contrary to some claims, no model portrayed the experimentally
observed inflection points.
 
Publisher Springer Science
 
Date 2000
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/842/1/patnaik2000.pdf
Patnaik, P R (2000) An evaluation of models for the effect of plasmid copy number on bacterial growth rate. Biotechnology Letters, 22 (21). pp. 1719-1725. ISSN 01415492
 
Relation http://dx.doi.org/10.1023/A:1005696401254
http://crdd.osdd.net/open/842/