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Evidence for plasmid-mediated chemotaxis of Pseudomonas putida towards naphthalene and salicylate.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Evidence for plasmid-mediated chemotaxis of Pseudomonas putida towards naphthalene and salicylate.
 
Creator Samanta, S K
Jain, R K
 
Subject QR Microbiology
 
Description A naphthalene (Nap) and salicylate (Sal) degrading microorganism, Pseudomonas putida RKJ1, is chemotactic towards these compounds. This strain carries a 83 kb plasmid. A 25 kb EcoRI fragment of the plasmid contains the genes responsible for Nap degradation through Sal. RKJ5, the plasmid-cured derivative of RKJ1, is neither capable of degradation nor is chemotactic towards Nap or Sal. The recombinant plasmid pRKJ3, which contained a 25 kb EcoRI fragment, was transferred back into the plasmid-free wild-type strain RKJ5, and the transconjugant showed both degradation and chemotaxis. The recombinant plasmid pRKJ3 was also transferred into motile, plasmid-free P. putida KT2442. The resulting transconjugant (RKJ15) showed chemotaxis towards both Nap and Sal. Two mutant strains carrying deletions in pRKJ3 (in KT2442) with phenotypes Nap- Sal+ and Nap- Sal-, were also tested for chemotaxis. It was found that the Nap- Sal+ mutant strain showed chemotaxis towards Sal only, whereas the Nap- Sal- mutant strain is non-chemotactic towards both the compounds. These results suggest that the metabolism of Nap and Sal may be required for the chemotactic activity.
 
Publisher National Research Council
 
Date 2000-01
 
Type Article
PeerReviewed
 
Identifier Samanta, S K and Jain, R K (2000) Evidence for plasmid-mediated chemotaxis of Pseudomonas putida towards naphthalene and salicylate. Canadian journal of microbiology, 46 (1). pp. 1-6. ISSN 0008-4166
 
Relation http://crdd.osdd.net/open/850/