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Plasmid stability analysis in a non-homogeneous bioreactor for a recombinant fed-batch fermentation

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Plasmid stability analysis in a non-homogeneous bioreactor for a recombinant fed-batch fermentation
 
Creator Patnaik, P R
 
Subject QD Chemistry
 
Description model incorporating segregational loss of the plasmid pPLc23rrpAl and imperfect macromixing of the broth. These
features become significant in large fermentation vessels, where fluid circulation in the bioreactor influences the rates of
cell growth and productivity. A simple model consisting of two interacting reactors describes the degree of macromixing,
which is characterized by the respective (internal) dilution rates. Controlled imperfect macromixing is superior to other
methods in arresting, and even reversing, the normal decline in the concentration of recombinant cells, thereby providing
a method to exploit the non-homogeneity of the broth in large bioreactors. Some physical implications are discussed.
On a analyst la fermentation en alimentation discontinue pour la synthttase de tryptophan par une souche de
Escherichia coli recombinante au moyen d’un modkle incorporant la perte stgrkgationnelle du plasmide pPLc23ttpA I
et le macromklange imparfait du bouillon. Ces caracttristiques deviennent significatives dans les rtservoirs de fermentation
de grande taille, oh la circulation des fluides dans le biorkacteur influence la vitesse de croissance et la productivitt
des cellules. Un modble simple composk de deux rtacteurs qui interagissent dtcrit le degrt de macromtlange, qui est
caracttrisk par les vitesses de dilution respectives (internes). Le macromklange imparfait contr6lk est suptrieur ?I celui
d’autres mtthodes pour stopper, et m&me inverser, le dkclin normal dans la concentration de cellules recombinantes, ce
qui fournit une mtthode pour exploiter la non-homogkntitt du bouillon dans les grands biortacteurs. Certaines implications
physiques sont analyskes.
 
Publisher Wiley
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/853/1/patnaik1999.7.pdf
Patnaik, P R (1999) Plasmid stability analysis in a non-homogeneous bioreactor for a recombinant fed-batch fermentation. The Canadian Journal of Chemical Engineering, 77. pp. 602-607. ISSN 00084034
 
Relation http://onlinelibrary.wiley.com/doi/10.1002/cjce.5450770325/pdf
http://crdd.osdd.net/open/853/