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Investigation of induction effect on the steady state performance of a continuous fermentation for recombinant β-ga1actosidase

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Investigation of induction effect on the steady state performance of a continuous fermentation for recombinant β-ga1actosidase
 
Creator Patnaik, P R
 
Subject QD Chemistry
 
Description The performance of continuousfermentation for an induced recombinant protein depends on the concentration of the inducer and on the inflow stream. The latter is characterized by the substrate concentration and the dilution rate, of which the latter is preferred as the manipulated variable for control. This work studies the effects of inducer (isopropyl-β-d-thiogalactopyranoside, IPTG) concentration and dilution rate on the steadystateperformance of acontinuousfermentation for β-galactosidase production by Escherichia coli containing the plasmid pUR2921. A low concentration of IPTG favours the growth of both viable (recombinant) cells and nonviable cells. To promote preferential growth of the former, their mass fraction should be maximized, and this requires a larger, optimal IPTG concentration. The expressed concentration of β-galactosidase passes through maxima with respect to both IPTG concentration and the dilution rate, whereas the viable cell concentration attains a peak only with respect to the dilution rate. While the optimal IPTG concentrations for mass fraction and for β-galactosidase are widely different, the two dilution rates are not far apart. This suggests two-stage fermentation at an intermediate dilution rate and different concentrations of IPTG.
 
Publisher Elsevier Science
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/868/1/patnaik2001.3.pdf
Patnaik, P R (2001) Investigation of induction effect on the steady state performance of a continuous fermentation for recombinant β-ga1actosidase. Process Biochemistry, 36 (11). pp. 1069-1074. ISSN 13595113
 
Relation http://dx.doi.org/10.1016/S0032-9592(01)00147-9
http://crdd.osdd.net/open/868/