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Synthesis of enhanced biosurfactant by Bacillus subtilis MTCC 2423 at 45°C by foam fractionation

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Synthesis of enhanced biosurfactant by Bacillus subtilis MTCC 2423 at 45°C by foam fractionation
 
Creator Makkar, R S
Cameotra, Swaranjit Singh
 
Subject QR Microbiology
 
Description fermentor was carried out using Bacillus subtilis
MTCC 2423 at 45°C. A good yield was obtained from sucrose
(2%) substrate fermentation by continuous removal of the
product by foam fractionation. The biosurfactant was recovered from collapsed foam by acid precipitation. The biosurfactant yield (4.5 g/L) was about 4.5 times higher than the yield (ca. 1 g/L) obtained by shake-flask fermentation. Surface activity of the collapsed foam was very high, and total surface activity was observed in the collapsed foam. The structural characterization
of this biosurfactant produced at 45°C by the strain used
in this study was recently reported. The biosurfactant was analyzed by thin-layer chromatography, infrared, 1H nuclear
magnetic resonance, and mass spectroscopy and was found
to be identical to surfactin, a lipopeptide surfactant.
 
Publisher Springer Science
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/875/1/swaranjit2001.pdf
Makkar, R S and Cameotra, Swaranjit Singh (2001) Synthesis of enhanced biosurfactant by Bacillus subtilis MTCC 2423 at 45°C by foam fractionation. Journal of Surfactants and Detergents, 4 (4). pp. 355-357. ISSN 1097-3958
 
Relation http://dx.doi.org/10.1007/s11743-001-0187-z
http://crdd.osdd.net/open/875/