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Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal
 
Creator Saha, Satish
Raje, Manoj
Suri, C Raman
 
Subject QR Microbiology
 
Description A multi-channel sandwich microgravimetric immunoassay (sMIA), using the quartz crystal microbalance (QCM)
principle, has been developed to quantify low molecular weight substances in standard solutions. An antigen is
sandwiched between two antigen-specific antibodies: the first antibody is coated on the quartz crystal surface and
the second antibody is used for the detection of analyte. The concentration of low molecular weight antigen (insulin
was used in this study, Mr ∼ 6000 Da) was correlated with the shift of resonant frequency of QCM system before
and after second antibody binding to insulin. The developed assay is highly specific showing low cross-reactivity,
and is sensitive to approx. 1 ng insulin ml−1 with a linear response for insulin from 10 μg ml−1 to 10 ng ml−1 in
standard solutions. The technique may also be applied for the detection of other small biomolecules
 
Publisher Springer Science
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/902/1/suri2002.pdf
Saha, Satish and Raje, Manoj and Suri, C Raman (2002) Sandwich microgravimetric immunoassay: Sensitive and specific detection of low molecular weight analytes using piezoelectric quartz crystal. Biotechnology Letters, 24 (9). pp. 711-716. ISSN 01415492
 
Relation http://dx.doi.org/10.1023/A:1015238201367
http://crdd.osdd.net/open/902/