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Steady state performance and sensitivity of a continuous recombinant fermentation with plasmid copy number multiplicity.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Steady state performance and sensitivity of a continuous recombinant fermentation with plasmid copy number multiplicity.
 
Creator Patnaik, P R
 
Subject QR Microbiology
 
Description Fermentations utilizing genetically modified cells usually contain two or more fractions of plasmid-bearing cells differing in the plasmid copy number. Competition among these cells and those without the plasmid may lead to a different performance than in the more 'ideal' situation where all recombinant cells have the same number of plasmids. A recent study considered the steady state sensitivities to the feed concentration of substrate and the dilution rate for a continuous fermentation with Saccharomyces cerevisiae XK1-C2 harbouring the plasmid pSXR125, which synthesizes b-galactosidase, with a uniform copy number. A similar analysis has been applied here to the same fermentation with a binomial distribution in the copy number. Although the steady states and their sensitivities are different from those for a uniform distribution, the dilution rate is still the favoured manipulated variable for control. While a selective medium generates a superior performance, its higher sensitivities require rapid on-line monitoring and anticipatory control.
 
Publisher NISCAIR
 
Date 2002
 
Type Article
PeerReviewed
 
Relation http://www.niscair.res.in/sciencecommunication/researchjournals/rejour/ijbt/ijbt-july2k2.htm#a4a
http://crdd.osdd.net/open/908/
 
Identifier Patnaik, P R (2002) Steady state performance and sensitivity of a continuous recombinant fermentation with plasmid copy number multiplicity. Indian Jouranl of Biotechnology, 1. pp. 263-269.