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Production and characterization of a thermostable beta-galactosidase from Bacillus coagulans RCS3.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Production and characterization of a thermostable beta-galactosidase from Bacillus coagulans RCS3.
 
Creator Batra, Navneet
Singh, Jagtar
Banerjee, U C
Patnaik, P R
Sobti, Ranbir C
 
Subject QR Microbiology
 
Description A strain of Bacillus coagulans RCS3 isolated from a hot-water spring produced significant beta-galactosidase activity at 10 days of growth in a flask. While enzyme production was maximum at 50 degrees C, the highest activity was at 65 degrees C, where the half-life was 2 h. A 2 degrees C decrease in temperature increased the half-life to 15 h without significantly changing the activity, suggesting that 63 degrees C is the temperature of preference compared with 65 degrees C for a combination of good activity and stability. The beta-galactosidase was also stable over pH 5-8, with peak activity at pH 6-7. It was strongly and competitively inhibited by the hydrolysis product galactose. Bivalent cations (Cu(2+), Ni(2+) and Hg(2+)) in the concentration range of 0.5-2.0 mM also inhibited enzyme activity. Both lactose solution and whey could be hydrolysed substantially within 36 h at 50 degrees C. The thermostability and pH-stability and good hydrolytic capability make this enzyme potentially useful in the dairy industry.
 
Publisher Portland Press
 
Date 2002
 
Type Article
PeerReviewed
 
Relation http://www.babonline.org/bab/036/bab0360001.htm
http://crdd.osdd.net/open/909/
 
Identifier Batra, Navneet and Singh, Jagtar and Banerjee, U C and Patnaik, P R and Sobti, Ranbir C (2002) Production and characterization of a thermostable beta-galactosidase from Bacillus coagulans RCS3. Biotechnology and applied biochemistry, 36 (Pt 1). pp. 1-6. ISSN 0885-4513