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Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p.
 
Creator Subba Rao, G
Bachhawat, Anand K
Gupta, C M
 
Subject QR Microbiology
 
Description The ATP-binding cassette (ABC) transporters are a large family of proteins responsible for the translocation of a variety of compounds across the membranes of both prokaryotes and eukaryotes. The inter-protein and intra-protein interactions in these traffic ATPases are still only poorly understood. In the present study we describe, for the first time, an extensive yeast two-hybrid (Y2H)-based analysis of the interactions of the cytoplasmic loops of the yeast pleiotropic drug resistance (Pdr) protein, Pdr5p, an ABC transporter of Saccharomyces cerevisiae. Four of the major cytosolic loops that have been predicted for this protein [including the two nucleotide-binding domain (NBD)-containing loops and the cytosolic C-terminal region] were subjected to an extensive inter-domain interaction study in addition to being used as baits to identify potential interacting proteins within the cell using the Y2H system. Results of these studies have revealed that the first cytosolic loop (CL1)--containing the first NBD domain--and also the C-terminal region of Pdr5p, interact with several candidate proteins. The possibility of an interaction between the CL1 loops of two neighboring Pdr5p molecules was also indicated, which could possibly have implications for dimerization of this protein.
 
Publisher Springer Science
 
Date 2002-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/914/1/bachhawat2002.pdf
Subba Rao, G and Bachhawat, Anand K and Gupta, C M (2002) Two-hybrid-based analysis of protein-protein interactions of the yeast multidrug resistance protein, Pdr5p. Functional & integrative genomics, 1 (6). pp. 357-66. ISSN 1438-793X
 
Relation http://www.springerlink.com/content/g115tnlh0vkcl2w3/fulltext.pdf
http://crdd.osdd.net/open/914/