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Utilization of glutathione as an exogenous sulfur source is independent of gamma-glutamyl transpeptidase in the yeast Saccharomyces cerevisiae: evidence for an alternative gluathione degradation pathway.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Utilization of glutathione as an exogenous sulfur source is independent of gamma-glutamyl transpeptidase in the yeast Saccharomyces cerevisiae: evidence for an alternative gluathione degradation pathway.
 
Creator Kumar, Chitranshu
Sharma, Rakesh
Bachhawat, Anand K
 
Subject QR Microbiology
 
Description gamma-Glutamyl transpeptidase (gamma-GT) is the only enzyme known to be responsible for glutathione degradation in living cells. In the present study we provide evidence that the utilization of glutathione can occur in the absence of gamma-GT. When disruptions in the CIS2 gene encoding gamma-GT were created in met15Delta strains, which require organic sulfur sources for growth, the cells were able to grow well with glutathione as the sole sulfur source suggesting that a gamma-GT-independent pathway for glutathione degradation exists in yeast cells. The CIS2 gene was strongly repressed by ammonium and derepressed in glutamate medium, and was found to be regulated by the nitrogen regulatory circuit. The utilization of glutathione as a sulfur source was, however, independent of the nitrogen source in the medium, further underlining that the two degradatory pathways were distinct.
 
Publisher Elsevier/North Holland on behalf of the Federation of European Microbiological Societies.
 
Date 2003-02-28
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/942/1/bachhawat2003.pdf
Kumar, Chitranshu and Sharma, Rakesh and Bachhawat, Anand K (2003) Utilization of glutathione as an exogenous sulfur source is independent of gamma-glutamyl transpeptidase in the yeast Saccharomyces cerevisiae: evidence for an alternative gluathione degradation pathway. FEMS microbiology letters, 219 (2). pp. 187-94. ISSN 0378-1097
 
Relation http://onlinelibrary.wiley.com/doi/10.1016/S0378-1097(03)00059-4/pdf
http://crdd.osdd.net/open/942/