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Effect of nutrients on optimal production of biosurfactants by Pseudomonas putida—A gujarat oil field isolate

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Effect of nutrients on optimal production of biosurfactants by Pseudomonas putida—A gujarat oil field isolate
 
Creator Pruthi, V.
Cameotra, Swaranjit Singh
 
Subject QR Microbiology
 
Description Nutritional requirements for maximal production
of biosurfactant by an oil field bacterium Pseudomonas
putida were determined. The optimal concentrations of nitrogen,phosphate, sulfur, magnesium, iron, potassium, sodium,calcium, and trace elements for maximal production of biosurfactants were ascertained, and a new “Pruthi and Cameotra” salt medium was formulated. Data show that maximal biomass (2.4 g L−1) and biosurfactant production (6.28 g L−1) takes place after 72 h of growth on 2% hexadecane. The biosurfactant was produced optimally over pH and temperature ranges of 6.4–7.2 and 30–40°C, respectively. That the highest biosurfactant yield was obtained during late log phase of growth indicates that the biosurfactant is a secondary metabolite.
 
Publisher Springer Science
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/945/1/swaranjit2003.pdf
Pruthi, V. and Cameotra, Swaranjit Singh (2003) Effect of nutrients on optimal production of biosurfactants by Pseudomonas putida—A gujarat oil field isolate. Journal of Surfactants and Detergents, 6 (1). pp. 65-68. ISSN 1097-3958
 
Relation http://dx.doi.org/10.1007/s11743-003-0250-9
http://crdd.osdd.net/open/945/