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A novel family of transporters mediating the transport of glutathione derivatives in plants.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title A novel family of transporters mediating the transport of glutathione derivatives in plants.
 
Creator Zhang, Ming-Yong
Bourbouloux, Andrée
Cagnac, Olivier
Srikanth, Chittur V
Rentsch, Doris
Bachhawat, Anand K
Delrot, Serge
 
Subject QR Microbiology
 
Description Uptake and compartmentation of reduced glutathione (GSH), oxidized glutathione (GSSG), and glutathione conjugates are important for many functions including sulfur transport, resistance against biotic and abiotic stresses, and developmental processes. Complementation of a yeast (Saccharomyces cerevisiae) mutant (hgt1) deficient in glutathione transport was used to characterize a glutathione transporter cDNA (OsGT1) from rice (Oryza sativa). The 2.58-kb full-length cDNA (AF393848, gi 27497095), which was obtained by screening of a cDNA library and 5'-rapid amplification of cDNA ends-polymerase chain reaction, contains an open reading frame encoding a 766-amino acid protein. Complementation of the hgt1 yeast mutant strain with the OsGT1 cDNA restored growth on a medium containing GSH as the sole sulfur source. The strain expressing OsGT1 mediated [3H]GSH uptake, and this uptake was significantly competed not only by unlabeled GSSG and GS conjugates but also by some amino acids and peptides, suggesting a wide substrate specificity. OsGT1 may be involved in the retrieval of GSSG, GS conjugates, and nitrogen-containing peptides from the cell wall.
 
Publisher American Society of Plant Biologists
 
Date 2004-01
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/957/1/bachhawat2004.pdf
Zhang, Ming-Yong and Bourbouloux, Andrée and Cagnac, Olivier and Srikanth, Chittur V and Rentsch, Doris and Bachhawat, Anand K and Delrot, Serge (2004) A novel family of transporters mediating the transport of glutathione derivatives in plants. Plant physiology, 134 (1). pp. 482-91. ISSN 0032-0889
 
Relation http://www.plantphysiol.org/content/134/1/482.full.pdf+html
http://crdd.osdd.net/open/957/