Record Details

Unusual emergence of guate98-like molecular subtype of DEN-3 during 2003 dengue outbreak in Delhi

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Unusual emergence of guate98-like molecular subtype of DEN-3 during 2003 dengue outbreak in Delhi
 
Creator Kumar, Manoj
Pasha, S.T
Mittal, V.
Arya, S.C.
Agrawal, N.
Bhattacharya, D.
Shiv, Lal
Rai, Arvind
 
Subject R Medicine (General)
 
Description With a view to identifying the molecular subtype of the circulating dengue virus responsible for a major
outbreak of dengue fever (DF) / dengue haemorrhagic fever (DHF) in and around Delhi during the postmonsoon
period in 2003, 32 serum samples were collected from clinically suspected cases. These were
subjected to reverse transcription/polymerase chain reaction (RT/PCR) for amplification of 511 bp CPreM
gene region of the dengue virus. Seven specimens, yielding a satisfactory quantum of viral RNA,
were subsequently processed for automated nucleotide sequencing. Five of the seven analysed isolates
showed close DNA sequence homology with Guate96-98 strains of DEN-3 virus, whereas two turned out
to be genotype IV of DEN-2. Earlier, DEN-2 (genotype IV) had been identified as the etiological agent
during a major DF/DHF outbreak in Delhi in 1996 and also in 2000. Though DEN-2 continues to prevail,
DEN-3, having a close sequence homology with Guate96-98 strains, seems to have entered India for the
first time in late 2003, resulting in a major DF/DHF outbreak. How the Guate96-98 strain of DEN-3
entered India remains to be linked epidemiologically.
 
Publisher WHO
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/966/1/manoj2004.1.pdf
Kumar, Manoj and Pasha, S.T and Mittal, V. and Arya, S.C. and Agrawal, N. and Bhattacharya, D. and Shiv, Lal and Rai, Arvind (2004) Unusual emergence of guate98-like molecular subtype of DEN-3 during 2003 dengue outbreak in Delhi. Dengue Bulletin, 28. pp. 457-461.
 
Relation http://www.searo.who.int/LinkFiles/Dengue_chapter_19.pdf
http://crdd.osdd.net/open/966/