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Optimal mixing to improve the performance of batch and continuous fermentations with recombinantEscherichia coli

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Optimal mixing to improve the performance of batch and continuous fermentations with recombinantEscherichia coli
 
Creator Ganduri, V.S.R.K.
Ghosh, Sanjoy
Patnaik, P R
 
Subject TP Chemical technology
 
Description Fermentations with genetically altered bacteria tend to lose plasmids as the fermentation
progresses. Methods such as two-stage cultivation, cell recycle and the addition of antibiotics are
commonly used to enhance plasmid stability. Here we examine a different method, the regulation of
mixing in the bioreactor. In particular, large bioreactors are considered where uniform mixing is difficult
to achieve and the probability of plasmid loss varies with the specific growth rate. For both batch and
continuous cultivations of Escherichia coli C600 gal K containing the plasmid pBR Eco gap, it is seen
through a model that bothmodes of operation exhibit high plasmid stability and cell growth when the broth
is incompletely mixed, and mixing near and away from the point of inoculation are unequal. Thus, the
natural incomplete mixing in large bioreactors may be utilized to improve plasmid stability. A practical
method to implement this idea is suggested.
 
Publisher wiley/Society of Chemical Industry
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/988/1/patnaik2005.pdf
Ganduri, V.S.R.K. and Ghosh, Sanjoy and Patnaik, P R (2005) Optimal mixing to improve the performance of batch and continuous fermentations with recombinantEscherichia coli. Journal of Chemical Technology & Biotechnology, 80 (4). pp. 361-370. ISSN 0268-2575
 
Relation http://dx.doi.org/10.1002/jctb.1194
http://crdd.osdd.net/open/988/