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Novel lectins from rhizomes of two Acorus species with mitogenic activity and inhibitory potential towards murine cancer cell lines.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Novel lectins from rhizomes of two Acorus species with mitogenic activity and inhibitory potential towards murine cancer cell lines.
 
Creator Bains, Jagmohan Singh
Dhuna, Vikram
Singh, Jatinder
Kamboj, Sukhdev Singh
Nijjar, Kamaljeet Kaur
Agrewala, J N
 
Subject QR180 Immunology
 
Description Two novel lectins were purified from rhizomes of two sweet flag species, namely Acorus calamus (Linn.) and Acorus gramineus (Solandin Ait.) by affinity chromatography on mannose linked epoxy-activated Sepharose 6B. The apparent molecular mass of the lectins, as determined by gel filtration chromatography, was 56 kDa for ACL and 55 kDa for AGL. In SDS-PAGE, pH 8.3, both lectins migrated with a subunit molecular mass of 13.6 kDa and 13.5 kDa, respectively, under reducing and non-reducing conditions thus indicating the absence of disulphide linkages. Acorus lectins readily agglutinated rabbit, rat and guinea pig erythrocytes. Both ACL and AGL also reacted with RBCs from sheep, goat and human ABO blood groups after neuraminidase treatment. ACL and AGL were inhibited by mannose/glucose and their derivatives. The most effective inhibitor was methyl-alpha-D-mannopyranoside. Acorus lectins were stable up to 55 degrees C, did not require metal ions for their activity and were also affected by high concentrations of denaturants like urea, thiourea and guanidine-HCl. These lectins showed potent mitogenic activity towards mouse splenocytes and human lymphocytes. Both ACL and AGL also significantly inhibited the growth of J774, a murine macrophage cancer cell-line and to lesser extent WEHI-279, a B-cell lymphoma.
 
Publisher Elsevier Science
 
Date 2005-08
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/993/1/agrewala2005.pdf
Bains, Jagmohan Singh and Dhuna, Vikram and Singh, Jatinder and Kamboj, Sukhdev Singh and Nijjar, Kamaljeet Kaur and Agrewala, J N (2005) Novel lectins from rhizomes of two Acorus species with mitogenic activity and inhibitory potential towards murine cancer cell lines. International immunopharmacology, 5 (9). pp. 1470-8. ISSN 1567-5769
 
Relation http://www.sciencedirect.com/science/article/pii/S1567576905001025
http://crdd.osdd.net/open/993/