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A mini-scale method for assay of D-amino acid oxidase activity inTrigonopsis variabilisagainst cephalosporin C

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title A mini-scale method for assay of D-amino acid oxidase activity inTrigonopsis variabilisagainst cephalosporin C
 
Creator Sonawane, Vijay Chintaman
 
Subject QR Microbiology
 
Description A mini-scale method for the estimation of D-amino acid oxidase activity against cephalosporin C was developed. The method allows implementation of the oxygenation and thorough mixing in a microfuge tube scale reactor. Dilution of reaction products in a buffer containing 0.03% hydrogen peroxide converted the intermediate product of the enzymatic reaction, α-keto adipyl-7-aminocephalosopranic acid (KA-7-ACA) to glutaryl-7-aminocephalosporanic acid (GL-7-ACA). In combination with high performance liquid chromatography, D-amino acid oxidase activity in the whole cells of Trigonopsis variabilis could be measured based on cephalosporin consumed in the reaction or the GL-7-ACA formed. This method could also be used for assay of D-amino acid oxidase activity in crude and soluble preparations
 
Publisher Taylor and francis group
 
Date 2005
 
Type Article
PeerReviewed
 
Relation http://dx.doi.org/10.1080/10242420500132417
http://crdd.osdd.net/open/996/
 
Identifier Sonawane, Vijay Chintaman (2005) A mini-scale method for assay of D-amino acid oxidase activity inTrigonopsis variabilisagainst cephalosporin C. Biocatalysis and Biotransformation, 23 (2). pp. 117-121. ISSN 1024-2422