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A novel antiproliferative and antifungal lectin from Amaranthus viridis Linn seeds.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title A novel antiproliferative and antifungal lectin from Amaranthus viridis Linn seeds.
 
Creator Kaur, Navjot
Dhuna, Vikram
Kamboj, Sukhdev Singh
Agrewala, J N
Singh, Jatinder
 
Subject QR Microbiology
 
Description A lectin from the seeds of Amaranthus viridis Linn has been purified by affinity chromatography on asialofetuin-linked amino activated silica. Amaranthus viridis lectin (AVL) has a native molecular mass of 67 kDa. It is a homodimer composed of two 36.6 kDa subunits. The lectin gave a single band in non-denaturing PAGE at pH 4.5 and pH 8.3 and a single peak on HPLC size exclusion and cation exchange columns. The purified lectin was specific for both T-antigen and N-acetyl-D-lactosamine, markers for various carcinomas, in addition to N-acetyl-D-galactosamine, asialofetuin and fetuin. This lectin reacted strongly with red blood cells (RBCs) from human ABO blood groups and rat. It also reacted with rabbit, sheep, goat and guinea pig RBCs. The lectin is a glycoprotein having no metal ion requirement for its activity. Denaturing agents such as urea, thiourea and guanidine-HCl had no effect on its activity when treated for 15 minutes. AVL showed significant antiproliferative activity towards HB98 and P388D1 murine cancer cell lines. It also exerted antifungal activity against phytopathogenic fungi Botrytis cincerea and Fusarium oxysporum but not against Rhizoctonia solani, Trichoderma reesei, Alternaria solani and Fusarium graminearum.
 
Publisher Bentham Science
 
Date 2006
 
Type Article
PeerReviewed
 
Relation http://www.benthamdirect.org/pages/content.php?PPL/2006/00000013/00000009/0005E.SGM
http://crdd.osdd.net/open/1019/
 
Identifier Kaur, Navjot and Dhuna, Vikram and Kamboj, Sukhdev Singh and Agrewala, J N and Singh, Jatinder (2006) A novel antiproliferative and antifungal lectin from Amaranthus viridis Linn seeds. Protein and peptide letters, 13 (9). pp. 897-905. ISSN 0929-8665