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PCR-based identification and strain typing of Pichia anomala using the ribosomal intergenic spacer region IGS1

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title PCR-based identification and strain typing of Pichia anomala using the ribosomal intergenic spacer region IGS1
 
Creator Bhardwaj, S.
Sutar, R.
Bachhawat, Anand K
Singhi, Sunit
Chakrabarti, Arunaloke
 
Subject R Medicine (General)
 
Description Frequent outbreaks of Pichia anomala fungaemia in paediatric patients have warranted the
development of a rapid identification system for this organism. This study describes a specific
PCR-based method targeting the rRNA gene intergenic spacer region 1 (IGS1) for rapid
identification of Pichia anomala isolates and characterization at the strain level. These methods of
species identification and strain typing were used on 106 isolates of Pichia anomala (77 from a
previously described outbreak and 29 isolated post-outbreak from the same hospital). Using
conventional morphological and biochemical methods, 11 strains isolated during the outbreak were
misidentified as P. anomala. BLAST analysis of sequences of internal transcribed spacer (ITS)
regions of rRNA genes confirmed that they were Pichia guilliermondii (eight isolates) and
Debaryomyces hansenii (three isolates). Strain typing of Pichia anomala isolates confirmed the
previous finding of a point-source outbreak. The results suggest that IGS sequences and their
polymorphisms could be exploited for similar typing methods in other organisms.
 
Publisher Society for General Microbiology (SGM)
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/1054/1/bachhawat2007.pdf
Bhardwaj, S. and Sutar, R. and Bachhawat, Anand K and Singhi, Sunit and Chakrabarti, Arunaloke (2007) PCR-based identification and strain typing of Pichia anomala using the ribosomal intergenic spacer region IGS1. Journal of Medical Microbiology, 56 (2). pp. 185-189. ISSN 0022-2615
 
Relation http://dx.doi.org/10.1099/jmm.0.46790-0
http://crdd.osdd.net/open/1054/