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The Macrophage Cell Surface Glyceraldehyde-3-phosphate Dehydrogenase Is a Novel Transferrin Receptor

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title The Macrophage Cell Surface Glyceraldehyde-3-phosphate Dehydrogenase Is a Novel Transferrin Receptor
 
Creator Raje, Chaaya Iyengar
Kumar, S.
Harle, A.
Nanda, Jagpreet S
Raje, Manoj
 
Subject QD Chemistry
 
Description The reticuloendothelial system plays a major role in iron metabolism. Despite this, the manner in which macrophages handle iron remains poorly understood. Mammalian cells utilize transferrin-dependent mechanisms to acquire iron via transferrin receptors 1 and 2 (TfR1 and TfR2) by receptor-mediated endocytosis. Here, we show for the first time that the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is localized on human and murine macrophage cell surface. The expression of this surface GAPDH is regulated by the availability of iron in the medium. We further demonstrate that this GAPDH interacts with transferrin and the GAPDH-transferrin complex is subsequently internalized into the early endosomes. Our work sheds new light on the mechanisms involved in regulation of iron, vital for controlling numerous diseases and maintaining normal immune function. Thus, we propose an entirely new avenue for investigation with respect to transferrin uptake and regulation mechanisms in macrophages.
 
Publisher ASBMB
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/1059/1/raje2007.pdf
Raje, Chaaya Iyengar and Kumar, S. and Harle, A. and Nanda, Jagpreet S and Raje, Manoj (2006) The Macrophage Cell Surface Glyceraldehyde-3-phosphate Dehydrogenase Is a Novel Transferrin Receptor. Journal of Biological Chemistry, 282 (5). pp. 3252-3261. ISSN 00219258
 
Relation http://dx.doi.org/10.1074/jbc.M608328200
http://crdd.osdd.net/open/1059/