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Palladium@gold bimetallic nanostructures as peroxidase mimic for development of sensitive fluoroimmunoassay.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Palladium@gold bimetallic nanostructures as peroxidase mimic for development of sensitive fluoroimmunoassay.
 
Creator Nangia, Yogesh
Kumar, Bipul
Kaushal, Jyotsna
Suri, C Raman
 
Subject QR Microbiology
 
Description In this paper, gold nanoparticles coated with palladium dots (Pd@Au) bimetallic nanostructures have been reported to have a peroxidase like activity which is not found in their monometallic counterparts. Based on this finding, we have developed an immunoassay in which antibody-modified Pd@Au nanostructure catalyzes the dimerization of a fluorogenic substrate for peroxidase, 3-(4-dihydroxy phenyl) propionic acid (HPPA), to generate high fluorescence signal. Specific antibodies against bensulfuron-methyl were generated by using a well characterized bensulfuron-protein conjugate as an immunogen, and the assay was performed in a competitive immunoassay format where Pd@Au nanostructure was bound to secondary antibody to show the peroxidase like activity. The developed immunoassay exhibited an excellent sensitivity showing a dynamic response range from 0.001 to 100ngmL(-1) for herbicide bensulfuron-methyl with a detection limit of 0.01ngmL(-1) (n=3). The newly synthesized bimetallic nanostructure shows the advantages of low cost, easy synthesis and tunable catalytic activity, making it a promising substitution of enzyme peroxidase in different applications.
 
Publisher Elsevier Science
 
Date 2012-11-02
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/1216/1/suri2012.1.pdf
Nangia, Yogesh and Kumar, Bipul and Kaushal, Jyotsna and Suri, C Raman (2012) Palladium@gold bimetallic nanostructures as peroxidase mimic for development of sensitive fluoroimmunoassay. Analytica chimica acta, 751. pp. 140-5. ISSN 1873-4324
 
Relation http://www.sciencedirect.com/science/article/pii/S0003267012013165
http://crdd.osdd.net/open/1216/