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Functional characterization of a novel Mn(2+) dependent protein serine/threonine kinase KpnK, produced by Klebsiella pneumoniae strain MGH78578.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Functional characterization of a novel Mn(2+) dependent protein serine/threonine kinase KpnK, produced by Klebsiella pneumoniae strain MGH78578.
 
Creator Srinivasan, Vijaya Bharathi
Vaidyanathan, Vasanth
Mondal, Amitabha
Venkataramaiah, Manjunath
Rajamohan, Govindan
 
Subject QR Microbiology
 
Description Klebsiella pneumoniae MGH78578 contains ∼500 uncharacterized signaling proteins and in this study, we characterized the biological functions of a novel eukaryotic-like serine/threonine kinase; ESTK (KpnK). Studies demonstrated that KpnK undergoes autophosphorylation within the pH range 7.0-7.5 at 37°C in a time- and concentration- dependent manner, with Mn(2+) as its cofactor. The ΔkpnK mutant exhibited higher sensitivity to gastrointestinal and oxidative stresses. Deletion of kpnK resulted in a two to threefold increased susceptibility towards imipenem, cefepime, ceftriaxone and ceftazidime. Our study has provided overall evidence for the involvement of ESTK in regulating bacterial physiology, stress response and drug resistance. This report has unmasked the occurrence of Ser/Thr kinase mediated signaling for the first time in K. pneumoniae. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: KpnKphosphorylatesKpnK by protein kinase assay (View interaction).
 
Publisher Elsevier Science
 
Date 2012-11-02
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/1219/1/rajmohan2012.1.pdf
Srinivasan, Vijaya Bharathi and Vaidyanathan, Vasanth and Mondal, Amitabha and Venkataramaiah, Manjunath and Rajamohan, Govindan (2012) Functional characterization of a novel Mn(2+) dependent protein serine/threonine kinase KpnK, produced by Klebsiella pneumoniae strain MGH78578. FEBS letters, 586 (21). pp. 3778-86. ISSN 1873-3468
 
Relation http://www.sciencedirect.com/science/article/pii/S0014579312007144
http://crdd.osdd.net/open/1219/