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Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii.
 
Creator Biswas, Dipanwita
Datt, Manish
Aggarwal, Monika
Mondal, Alok K
 
Subject QR Microbiology
 
Description Because of its natural ability to utilize both xylose and arabinose, the halotolerant and osmotolerant yeast Debaryomyces hansenii is considered as a potential microbial platform for exploiting lignocellulosic biomass. To gain better understanding of the xylose metabolism in D. hansenii, we have cloned and characterized a xylitol dehydrogenase gene (DhXDH). The cloned gene appeared to be essential for xylose metabolism in D. hansenii as the deletion of this gene abolished the growth of the cells on xylose. The expression of DhXDH was strongly upregulated in the presence of xylose. Recombinant DhXdhp was expressed and purified from Escherichia coli. DhXdhp was highly active against xylitol and sorbitol as substrate. Our results showed that DhXdhp was thermo-sensitive, and except this, its biochemical properties were quite comparable with XDH from other yeast species. Furthermore, to make this enzyme suitable for metabolic engineering of D. hansenii, we have improved its thermotolerance and modified cofactor requirement through modelling and mutagenesis approach.
 
Publisher Springer
 
Date 2012-04-11
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/1251/1/mondal2012.pdf
Biswas, Dipanwita and Datt, Manish and Aggarwal, Monika and Mondal, Alok K (2012) Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii. Applied microbiology and biotechnology. ISSN 1432-0614
 
Relation http://link.springer.com/article/10.1007%2Fs00253-012-4020-5
http://crdd.osdd.net/open/1251/