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Rapid extraction and quantitative detection of the herbicide diuron in surface water by a hapten-functionalized carbon nanotubes based electrochemical analyzer.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Rapid extraction and quantitative detection of the herbicide diuron in surface water by a hapten-functionalized carbon nanotubes based electrochemical analyzer.
 
Creator Sharma, Priyanka
Bhalla, Vijayender
Tuteja, Satish
Kukkar, Manil
Suri, C Raman
 
Subject QR Microbiology
 
Description A solid phase extraction micro-cartridge containing a non-polar polystyrene absorbent matrix was coupled with an electrochemical immunoassay analyzer (EIA) and used for the ultra-sensitive detection of the phenyl urea herbicide diuron in real samples. The EIA was fabricated by using carboxylated carbon nanotubes (CNTs) functionalized with a hapten molecule (an amine functionalized diuron derivative). Screen printed electrodes (SPE) were modified with these haptenized CNTs and specific in-house generated anti diuron antibodies were used for bio-interface development. The immunodetection was realized in a competitive electrochemical immunoassay format using alkaline phosphatase labeled secondary anti-IgG antibody. The addition of 1-naphthyl phosphate substrate resulted in the production of an electrochemically active product, 1-naphthol, which was monitored by using differential pulse voltammetry (DPV). The assay exhibited excellent sensitivity and specificity having a dynamic response range of 0.01 pg mL(-1) to 10 μg mL(-1) for diuron with a limit of detection of around 0.1 pg mL(-1) (n = 3) in standard water samples. The micro-cartridge coupled hapten-CNTs modified SPE provided an effective and efficient electrochemical immunoassay for the real-time monitoring of pesticides samples with a very high degree of sensitivity.
 
Publisher RSC Publishing
 
Date 2012-05-21
 
Type Article
PeerReviewed
 
Relation http://pubs.rsc.org/en/Content/ArticleLanding/2012/AN/c2an16235k
http://crdd.osdd.net/open/1256/
 
Identifier Sharma, Priyanka and Bhalla, Vijayender and Tuteja, Satish and Kukkar, Manil and Suri, C Raman (2012) Rapid extraction and quantitative detection of the herbicide diuron in surface water by a hapten-functionalized carbon nanotubes based electrochemical analyzer. The Analyst, 137 (10). pp. 2495-502. ISSN 1364-5528