Record Details

Phenyl aldehyde and propanoids exert multiple sites of action towards cell membrane and cell wall targeting ergosterol in Candida albicans

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Phenyl aldehyde and propanoids exert multiple sites of action towards cell membrane and cell wall targeting ergosterol in Candida albicans
 
Creator Khan, Mohd Sajjad
Ahmad, Iqbal
Cameotra, Swaranjit Singh
 
Subject QR Microbiology
 
Description In the present study, two phyto-compounds phenyl aldehyde (cinnamaldehyde) and propanoid (eugenol) were
selected to explore their modes of action against Candida albicans. Electron microscopy, flow cytometry and
spectroscopic assays were employed to determine the targets of these compounds. Treatment of C. albicans (CA04)
with sub-MICs of cinnamaldehyde (50 μg/mL) and eugenol (200 μg/mL) indicated multiple sites of action including
damages to cell walls, cell membranes, cytoplasmic contents and other membranous structures as observed under
electron microscopy. Concentration and time dependent increase in the release of cytoplasmic contents
accompanied with change in extracellular K+ concentration was recorded. Exposure of Candida cells at 4 × MIC of
cinnaamldehyde and eugenol resulted in 40.21% and 50.90% dead cells, respectively as revealed by flow cytometry
analysis. Treatment of Candida cells by cinnamaldehyde and eugenol at 0.5 × MIC showed 67.41% and 76.23%
reduction in ergosterol biosynthesis, respectively. The binding assays reflected the ability of compounds to bind
with the ergosterol. Our findings have suggested that the membrane damaging effects of phenyl aldehyde and
propanoids class of compounds is attributed to their ability to inhibit ergosterol biosynthesis and simultaneously
binding with ergosterol. Indirect or secondary action of these compounds on cell wall is also expected as revealed
by electron microscopic studies.
 
Publisher Springer Science
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/1513/1/swaranjit14.pdf
Khan, Mohd Sajjad and Ahmad, Iqbal and Cameotra, Swaranjit Singh (2013) Phenyl aldehyde and propanoids exert multiple sites of action towards cell membrane and cell wall targeting ergosterol in Candida albicans. AMB Express, 3 (1). p. 54. ISSN 2191-0855
 
Relation http://dx.doi.org/10.1186/2191-0855-3-54
http://crdd.osdd.net/open/1513/