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Mycobacterium tuberculosis acquires iron by cell-surface sequestration and internalization of human holo-transferrin.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Mycobacterium tuberculosis acquires iron by cell-surface sequestration and internalization of human holo-transferrin.
 
Creator Boradia, Vishant Mahendra
Malhotra, Himanshu
Thakkar, Janak Shrikant
Tillu, Vikas Ajit
Vuppala, Bhavana
Patil, Pravinkumar
Sheokand, Navdeep
Sharma, Prerna
Chauhan, Anoop Singh
Raje, Manoj
Raje, Chaaya Iyengar
 
Subject QR Microbiology
 
Description Mycobacterium tuberculosis (M.tb), which requires iron for survival, acquires this element by synthesizing iron-binding molecules known as siderophores and by recruiting a host iron-transport protein, transferrin, to the phagosome. The siderophores extract iron from transferrin and transport it into the bacterium. Here we describe an additional mechanism for iron acquisition, consisting of an M.tb protein that drives transport of human holo-transferrin into M.tb cells. The pathogenic strain M.tb H37Rv expresses several proteins that can bind human holo-transferrin. One of these proteins is the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Rv1436), which is present on the surface of M.tb and its relative Mycobacterium smegmatis. Overexpression of GAPDH results in increased transferrin binding to M.tb cells and iron uptake. Human transferrin is internalized across the mycobacterial cell wall in a GAPDH-dependent manner within infected macrophages.
 
Publisher Nature Pub. Group
 
Date 2014
 
Type Article
PeerReviewed
 
Relation http://www.nature.com/ncomms/2014/140828/ncomms5730/full/ncomms5730.html
http://crdd.osdd.net/open/1530/
 
Identifier Boradia, Vishant Mahendra and Malhotra, Himanshu and Thakkar, Janak Shrikant and Tillu, Vikas Ajit and Vuppala, Bhavana and Patil, Pravinkumar and Sheokand, Navdeep and Sharma, Prerna and Chauhan, Anoop Singh and Raje, Manoj and Raje, Chaaya Iyengar (2014) Mycobacterium tuberculosis acquires iron by cell-surface sequestration and internalization of human holo-transferrin. Nature communications, 5. p. 4730. ISSN 2041-1723