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Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene.
 
Creator Sabherwal, Priyanka
Shorie, Munish
Pathania, Preeti
Chaudhary, Shilpa
Bhasin, K K
Bhalla, Vijayender
Suri, C Raman
 
Description Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.
 
Publisher ACS
 
Date 2014-08-05
 
Type Article
PeerReviewed
 
Relation http://pubs.acs.org/doi/abs/10.1021/ac501388a
http://crdd.osdd.net/open/1538/
 
Identifier Sabherwal, Priyanka and Shorie, Munish and Pathania, Preeti and Chaudhary, Shilpa and Bhasin, K K and Bhalla, Vijayender and Suri, C Raman (2014) Hybrid aptamer-antibody linked fluorescence resonance energy transfer based detection of trinitrotoluene. Analytical chemistry, 86 (15). pp. 7200-4. ISSN 1520-6882