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Isolation and functional analysis of a glycolipid producing Rhodococcus sp. strain IITR03 with potential for degradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT).

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Isolation and functional analysis of a glycolipid producing Rhodococcus sp. strain IITR03 with potential for degradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT).
 
Creator Bajaj, Abhay
Mayilraj, Shanmugam
Mudiam, Mohana Krishna Reddy
Patel, Devendra Kumar
Manickam, Natesan
 
Description A 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degrading bacterium strain IITR03 producing trehalolipid was isolated and characterized from a pesticides contaminated soil. The strain IITR03 was identified as a member of the genus Rhodococcus based on polyphasic studies. Under aqueous culture conditions, the strain IITR03 degraded 282 μM of DDT and could also utilize 10mM concentration each of 4-chlorobenzoic acid, 3-chlorobenzoic acid and benzoic acid as sole carbon and energy source. The catechol 1,2-dioxygenase enzyme activity resulted in conversion of catechol to form cis,cis-muconic acid. Cloning and sequencing of partial nucleotide sequence of catechol 1,2-dioxygenase gene (cat) from strain IITR03 revealed its similarity to catA gene present in Rhodococcus sp. strain Lin-2 (97% identity) and Rhodococcus strain AN22 (96% identity) degrading benzoate and aniline, respectively. The results suggest that the strain IITR03 could be useful for field bioremediation studies of DDT-residues and chlorinated aromatic compounds present in contaminated sites.
 
Publisher Elsevier
 
Date 2014-09
 
Type Article
PeerReviewed
 
Relation http://www.sciencedirect.com/science/article/pii/S0960852414008517
http://crdd.osdd.net/open/1539/
 
Identifier Bajaj, Abhay and Mayilraj, Shanmugam and Mudiam, Mohana Krishna Reddy and Patel, Devendra Kumar and Manickam, Natesan (2014) Isolation and functional analysis of a glycolipid producing Rhodococcus sp. strain IITR03 with potential for degradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT). Bioresource technology, 167. pp. 398-406. ISSN 1873-2976