Surfactin restores and enhances swarming motility under heavy metal stress.
DIR@IMTECH: CSIR-Institute of Microbial Technology
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Title |
Surfactin restores and enhances swarming motility under heavy metal stress.
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Creator |
Singh, Anil Kumar
Dhanjal, Soniya Cameotra, Swaranjit Singh |
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Description |
The present work reports the importance of lipopeptide biosurfactant on swarming motility of multi-metal resistant (MMR) bacterium under heavy metal stress. The MMR bacteria strain CM100B, identified as Bacillus cereus, was isolated from the coal mine sample. The strain was able to grow and reduce several metals namely Cd(2+), Co(2+), Cu(2+), Ni(2+), Mn(2+) and Pb(2+) ions which are common environmental pollutants. Presence of toxic heavy metal ions in the swarming medium significantly altered the motility of CM100B. Presence of Cd(2+) and Pb(2+) ions inhibited development of peritrichous flagella, thus inhibiting swarming motility. However, the addition of anionic biosurfactant surfactin restored (in case of Cd(2+) and Pb(2+) ions) or enhanced (in case of Co(2+), Cu(2+), Ni(2+) and Mn(2+)) the swarming ability of CM100B. Zeta potential studies for determining bacterial cell surface charge indicated that surfactin provided a suitable swarming environment to bacteria even under metal stress by chelating to cationic metal ions. Non-ionic surfactant Triton X-100 was unable to restore swarming under Cd(2+) and Pb(2+) ion stress. Thus, suggesting that surfactin can aid in motility not only by reducing the surface tension of swarming medium but also by binding to metal ions in the presence of metal ions stress.
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Publisher |
Amsterdam ; New York : Elsevier, c1993-
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Date |
2014-04-01
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Type |
Article
PeerReviewed |
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Relation |
http://www.sciencedirect.com/science/article/pii/S0927776513007881
http://crdd.osdd.net/open/1552/ |
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Identifier |
Singh, Anil Kumar and Dhanjal, Soniya and Cameotra, Swaranjit Singh (2014) Surfactin restores and enhances swarming motility under heavy metal stress. Colloids and surfaces. B, Biointerfaces, 116. pp. 26-31. ISSN 1873-4367
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