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Repurposing TRASH: emergence of the enzyme organomercurial lyase from a non-catalytic zinc finger scaffold.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Repurposing TRASH: emergence of the enzyme organomercurial lyase from a non-catalytic zinc finger scaffold.
 
Creator Kaur, Gurmeet
Subramanian, Srikrishna
 
Description The mercury resistance pathway enzyme organomercurial lyase (MerB) catalyzes the conversion of organomercurials to ionic mercury (Hg(2+)). Here, we provide evidence for the emergence of this enzyme from a TRASH-like, non-enzymatic, treble-clef zinc finger ancestor by domain duplication and fusion. Surprisingly, the structure-stabilizing metal-binding core of the treble-clef appears to have been repurposed in evolution to serve a catalytic role. Novel enzymatic functions are believed to have evolved from ancestral generalist catalytic scaffolds or from already specialized enzymes with catalytic promiscuity. The emergence of MerB from a zinc finger ancestor serves as a rare example of how a novel enzyme may emerge from a non-catalytic scaffold with a related binding function.
 
Publisher Elsevier
 
Date 2014-10
 
Type Article
NonPeerReviewed
 
Relation http://www.sciencedirect.com/science/article/pii/S1047847714001877
http://crdd.osdd.net/open/1561/
 
Identifier Kaur, Gurmeet and Subramanian, Srikrishna (2014) Repurposing TRASH: emergence of the enzyme organomercurial lyase from a non-catalytic zinc finger scaffold. Journal of structural biology, 188 (1). pp. 16-21. ISSN 1095-8657