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In vitro and in vivo antibacterial evaluation and mechanistic study of ornithine based small cationic lipopeptides against antibiotic resistant clinical isolates.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title In vitro and in vivo antibacterial evaluation and mechanistic study of ornithine based small cationic lipopeptides against antibiotic resistant clinical isolates.
 
Creator Lohan, Sandeep
Monga, Jitender
Cameotra, Swaranjit Singh
Bisht, Gopal Singh
 
Description We investigated the in vitro activities of short lipopeptides against a large panel of clinical isolates of antibiotic resistant bacteria. In the animal model, LP16 (5 mg/kg) significantly decreased the burden of viable colony forming unit (CFU) of bacteria. MTT assay results revealed the high selectivity of lipopeptides toward microbial cells. Calcein dye leakage experiments and flow cytometric analysis suggests the membranolytic effect of lipopeptides, which was further confirmed by visualizing bacterial damage via electron microscopy tool (SEM & TEM). Moreover, stability in human blood plasma and no sign of resistance development against clinical isolates of Escherichia coli and Staphylococcus aureus were observed for lead lipopeptides. These results demonstrate the potential of short lipopeptides as a novel class of anti-infectives.
 
Publisher Paris : Editions Scientifiques Elsevier
 
Date 2014-12-17
 
Type Article
PeerReviewed
 
Relation http://www.sciencedirect.com/science/article/pii/S0223523414005583
http://crdd.osdd.net/open/1571/
 
Identifier Lohan, Sandeep and Monga, Jitender and Cameotra, Swaranjit Singh and Bisht, Gopal Singh (2014) In vitro and in vivo antibacterial evaluation and mechanistic study of ornithine based small cationic lipopeptides against antibiotic resistant clinical isolates. European journal of medicinal chemistry, 88. pp. 19-27. ISSN 1768-3254