Record Details

Point-of-Care Amperometric Testing of Diabetic Marker (HbA1c) Using Specific Electroactive Antibodies

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Point-of-Care Amperometric Testing of Diabetic Marker (HbA1c) Using Specific Electroactive Antibodies
 
Creator Chopra, Adity
Rawat, Sanjay
Bhalla, Vijayender
Suri, C Raman
 
Subject QR Microbiology
 
Description Specific immune detection of glycated hemoglobin is still a great challenge owing to the small epitopic difference between Hemoglobin (Hb) and HbA1c. We report a new electrochemical immunoassay format for point of care testing of HbA1c. A conducting self-assembled monolayer of mercaptophenyl boronic acid (MPBA) was used as a capture layer for binding of glycated proteins and ferrocene tagged anti-HbA1c antibody (FcAb) as a tracer molecule on a gold screen printed electrode. Validation of the new HbA1c assay was carried out using 6 clinical samples with known HPLC values and a correlation coefficient of 98 % was observed.
 
Publisher Wiley
 
Date 2014
 
Type Article
PeerReviewed
 
Relation http://dx.doi.org/10.1002/elan.201300646
http://crdd.osdd.net/open/1706/
 
Identifier Chopra, Adity and Rawat, Sanjay and Bhalla, Vijayender and Suri, C Raman (2014) Point-of-Care Amperometric Testing of Diabetic Marker (HbA1c) Using Specific Electroactive Antibodies. Electroanalysis, 26 (3). pp. 469-472. ISSN 10400397