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Rhodotorula svalbardensis sp. nov., a novel yeast species isolated from cryoconite holes of Ny-Ålesund, Arctic.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Rhodotorula svalbardensis sp. nov., a novel yeast species isolated from cryoconite holes of Ny-Ålesund, Arctic.
 
Creator Singh, Purnima
Singh, Shiv M
Tsuji, Masaharu
Prasad, G S
Hoshino, Tamotsu
 
Subject QR Microbiology
 
Description A psychrophilic yeast species was isolated from glacier cryoconite holes of Svalbard. Nucleotide sequences of the strains were studied using D1/D2 domain, ITS region and partial sequences of mitochondrial cytochrome b gene. The strains belonged to a clade of psychrophilic yeasts, but showed marked differences from related species in the D1/D2 domain and biochemical characters. Effects of temperature, salt and media on growth of the cultures were also studied. Screening of the cultures for amylase, cellulase, protease, lipase, urease and catalase activities was carried out. The strains expressed high amylase and lipase activities. Freeze tolerance ability of the isolates indicated the formation of unique hexagonal ice crystal structures due to presence of 'antifreeze proteins' (AFPs). FAME analysis of cultures showed a unique trend of increase in unsaturated fatty acids with decrease in temperature. The major fatty acids recorded were oleic acid, linoleic acid, linolenic acid, palmitic acid, stearic acid, myristic acid and pentadecanoic acid. Based on sequence data and, physiological and morphological properties of the strains, we propose a novel species, Rhodotorula svalbardensis and designate strains MLB-I (CCP-II) and CRY-YB-1 (CBS 12863, JCM 19699, JCM 19700, MTCC 10952) as its type strains (Etymology: sval.bar.den'sis. N.L. fem. adj. svalbardensis pertaining to Svalbard).
 
Publisher Elsevier Publisher
 
Date 2014-02
 
Type Article
PeerReviewed
 
Relation http://www.sciencedirect.com/science/article/pii/S001122401400008X
http://crdd.osdd.net/open/1712/
 
Identifier Singh, Purnima and Singh, Shiv M and Tsuji, Masaharu and Prasad, G S and Hoshino, Tamotsu (2014) Rhodotorula svalbardensis sp. nov., a novel yeast species isolated from cryoconite holes of Ny-Ålesund, Arctic. Cryobiology, 68 (1). pp. 122-8. ISSN 1090-2392