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Secreted multifunctional Glyceraldehyde-3-phosphate dehydrogenase sequesters lactoferrin and iron into cells via a non-canonical pathway.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Secreted multifunctional Glyceraldehyde-3-phosphate dehydrogenase sequesters lactoferrin and iron into cells via a non-canonical pathway.
 
Creator Chauhan, Anoop S
Rawat, Pooja
Malhotra, Himanshu
Sheokand, Navdeep
Kumar(1), Manoj
Patidar, Anil
Chaudhary, Surbhi
Jakhar, Priyanka
Raje, Chaaya I
Raje, Manoj
 
Subject QR Microbiology
 
Description Lactoferrin is a crucial nutritionally important pleiotropic molecule and iron an essential trace metal for all life. The current paradigm is that living organisms have evolved specific membrane anchored receptors along with iron carrier molecules for regulated absorption, transport, storage and mobilization of these vital nutrients. We present evidence for the existence of non-canonical pathway whereby cells actively forage these vital resources from beyond their physical boundaries, by secreting the multifunctional housekeeping enzyme Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) into the extracellular milieu. This effect's an autocrine/paracrine acquisition of target ligand into the cell. Internalization by this route is extensively favoured even by cells that express surface receptors for lactoferrin and involves urokinase plasminogen activator receptor (uPAR). We also demonstrate the operation of this phenomenon during inflammation, as an arm of the innate immune response where lactoferrin denies iron to invading microorganisms by chelating it and then itself being sequestered into surrounding host cells by GAPDH.
 
Publisher NPG
 
Date 2015
 
Type Article
PeerReviewed
 
Relation http://www.nature.com/articles/srep18465
http://crdd.osdd.net/open/1733/
 
Identifier Chauhan, Anoop S and Rawat, Pooja and Malhotra, Himanshu and Sheokand, Navdeep and Kumar(1), Manoj and Patidar, Anil and Chaudhary, Surbhi and Jakhar, Priyanka and Raje, Chaaya I and Raje, Manoj (2015) Secreted multifunctional Glyceraldehyde-3-phosphate dehydrogenase sequesters lactoferrin and iron into cells via a non-canonical pathway. Scientific reports, 5. p. 18465. ISSN 2045-2322