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Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth.
 
Creator Haque, Farazul
Alfatah, Md
Ganesan, K
Bhattacharyya, Mani Shankar
 
Subject QR Microbiology
 
Description Candida albicans causes superficial and life-threatening systemic infections. These are difficult to treat often due to drug resistance, particularly because C. albicans biofilms are inherently resistant to most antifungals. Sophorolipid (SL), a glycolipid biosurfactant, has been shown to have antimicrobial and anticancer properties. In this study, we investigated the effect of SL on C. albicans biofilm formation and preformed biofilms. SL was found to inhibit C. albicans biofilm formation as well as reduce the viability of preformed biofilms. Moreover, SL, when used along with amphotericin B (AmB) or fluconazole (FLZ), was found to act synergistically against biofilm formation and preformed biofilms. Effect of SL on C. albicans biofilm formation was further visualized by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), which revealed absence of hyphae, typical biofilm architecture and alteration in the morphology of biofilm cells. We also found that SL downregulates the expression of hypha specific genes HWP1, ALS1, ALS3, ECE1 and SAP4, which possibly explains the inhibitory effect of SL on hyphae and biofilm formation.
 
Publisher NPG
 
Date 2016
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/1871/1/30.pdf
Haque, Farazul and Alfatah, Md and Ganesan, K and Bhattacharyya, Mani Shankar (2016) Inhibitory Effect of Sophorolipid on Candida albicans Biofilm Formation and Hyphal Growth. Scientific reports, 6. p. 23575. ISSN 2045-2322
 
Relation http://www.nature.com/articles/srep23575
http://crdd.osdd.net/open/1871/