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Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture.
 
Creator Priyanka, Anu
Solanki, Vipul
Parkesh, Raman
Thakur, Krishan Gopal
 
Subject QR Microbiology
 
Description Human SIRT7 is an NAD(+) dependent deacetylase, which belongs to sirtuin family of proteins. SIRT7, like other sirtuins has conserved catalytic domain and is flanked by N- and C-terminal domains reported to play vital functional roles. Here, we report the crystal structure of the N-terminal domain of human SIRT7 (SIRT7(NTD) ) at 2.3 Å resolution as MBP-SIRT7(NTD) fusion protein. SIRT7(NTD) adopts three-helical domain architecture and comparative structural analyses suggest similarities to some DNA binding motifs and transcription regulators. We also report here the importance of N- and C-terminal domains in soluble expression of SIRT7. Proteins 2016; 84:1558-1563. © 2016 Wiley Periodicals, Inc.
 
Publisher Wiley
 
Date 2016-10
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://crdd.osdd.net/open/1897/1/67.pdf
Priyanka, Anu and Solanki, Vipul and Parkesh, Raman and Thakur, Krishan Gopal (2016) Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture. Proteins, 84 (10). pp. 1558-63. ISSN 1097-0134
 
Relation http://onlinelibrary.wiley.com/doi/10.1002/prot.25085/abstract
http://crdd.osdd.net/open/1897/