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A label-free electrochemical immunosensor for the detection of cardiac marker using graphene quantum dots (GQDs).

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title A label-free electrochemical immunosensor for the detection of cardiac marker using graphene quantum dots (GQDs).
 
Creator Tuteja, Satish K
Chen, Rui
Kukkar, Manil
Song, Chung Kil
Mutreja, Ruchi
Singh, Suman
Paul, Ashok K
Lee, Haiwon
Kim, Ki-Hyun
Deep, Akash
Suri, C Raman
 
Subject QR Microbiology
 
Description A label-free immunosensor based on electrochemical impedance spectroscopy has been developed for the sensitive detection of a cardiac biomarker myoglobin (cMyo). Hydrothermally synthesized graphene quantum dots (GQDs) have been used as an immobilized template on screen printed electrodes for the construction of an impedimetric sensor platform. The GQDs-modified electrode was conjugated with highly specific anti-myoglobin antibodies to develop the desired immunosensor. The values of charge transfer resistance (Rct) were monitored as a function of varying antigen concentration. The Rct value of the immunosensor showed a linear increase (from 0.20 to 0.31kΩ) in the range of 0.01-100ng/mL cMyo. The specific detection of cMyo was also made in the presence of other competing proteins. The limit of detection for the proposed immunosensor was estimated as 0.01ng/mL which is comparable to the standard ELISA techniques.
 
Publisher Elsevier Science
 
Date 2016-12-15
 
Type Article
PeerReviewed
 
Relation http://linkinghub.elsevier.com/retrieve/pii/S0956-5663(16)30681-9
http://crdd.osdd.net/open/1909/
 
Identifier Tuteja, Satish K and Chen, Rui and Kukkar, Manil and Song, Chung Kil and Mutreja, Ruchi and Singh, Suman and Paul, Ashok K and Lee, Haiwon and Kim, Ki-Hyun and Deep, Akash and Suri, C Raman (2016) A label-free electrochemical immunosensor for the detection of cardiac marker using graphene quantum dots (GQDs). Biosensors & bioelectronics, 86. pp. 548-56. ISSN 1873-4235