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Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India.

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India.
 
Creator Kaur, Simerpreet
Rana, Vibhuti
Singh, Pooja
Trivedi, Garima
Anand, Shashi
Kaur, Amanpreet
Gupta, Pawan
Jain, Amita
Sharma, Charu
 
Subject QR Microbiology
 
Description Twenty-nine Kanamycin resistant clinical isolates of Mycobacterium tuberculosis from Northern India were screened to evaluate genetic mutations in rrs gene, eis gene with its promoter, and whiB7 gene along with its 5'UTR. 14 strains (~48.0%) collectively exhibited mutations in rrs, eis or whiB7 target regions. While the highest frequency of mutations was found in rrs gene, eis and whiB7 loci displayed novel mutations. The novel mutations displayed by eis and whiB7 loci were found to be associated specifically with the Kanamycin resistance as none of the twenty nine Kanamycin sensitive strains harbor them. The inclusion of novel mutations of eis and whiB7 loci will be useful in improving the specificity of future diagnostics.
 
Publisher Elsevier Science
 
Date 2016-01
 
Type Article
PeerReviewed
 
Relation http://www.sciencedirect.com/science/article/pii/S1472979215300639
http://crdd.osdd.net/open/1916/
 
Identifier Kaur, Simerpreet and Rana, Vibhuti and Singh, Pooja and Trivedi, Garima and Anand, Shashi and Kaur, Amanpreet and Gupta, Pawan and Jain, Amita and Sharma, Charu (2016) Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India. Tuberculosis (Edinburgh, Scotland), 96. pp. 96-101. ISSN 1873-281X