Record Details

Degradation kinetics of resorcinol by Enterobacter cloacae isolate

IR@CSIR-NEERI

View Archive Info
 
 
Field Value
 
Title Degradation kinetics of resorcinol by Enterobacter
cloacae isolate
 
Creator Jablonski, Marissa R
Shaligram, Shraddha
Qureshi, Asifa
Purohit, H J
Reisel, John R
 
Subject Environmental Pollution
Microbiology
 
Description Resorcinol was utilized as the sole carbon and energy source by Enterobacter cloacae (identification by 16S rDNA nucleotide sequencing Genbank Accession Number JN093148). The different concentration of resorcinol utilized by the bacterial isolate ranged between 55 and 220 mg l-1 at 30°C and pH of 7.0. It was observed that the batch experimental results were best fitted for Michaelis-Menten and Monod models (for 220 mg l-1 resorcinol) with time under defined conditions. The kinetics constants for the Michaelis-Menten equation (enzyme kinetics) were Km = 11.00 mM and Vmax = 0.03 mM min-1 and for the Monod equation (growth kinetics) was μmax = 0.0371 h-1 in the inhibitory region and KS = 22.09 mg l-1. It was assumed that enzyme reactions limit biomass production (Monod kinetics) during resorcinol degradation by E. cloacae. The enzyme kinetic model (Michaelis-Menten) used was fit to the resorcinol degradation profiles with a set of model parameters such as using pre-induced E. cloacae cells on 220 mg l-1 resorcinol.
 
Publisher Academic Journals
 
Date 2013-07-12
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/429/1/Asifa_AJMR2013_Jablonski_et_al.pdf
Jablonski, Marissa R and Shaligram, Shraddha and Qureshi, Asifa and Purohit, H J and Reisel, John R (2013) Degradation kinetics of resorcinol by Enterobacter cloacae isolate. African Journal of Microbiology Research, 7 (28). pp. 3632-3640. ISSN 1996-0808
 
Relation http://www.academicjournals.org/journal/AJMR/edition/12_July,_2013
http://neeri.csircentral.net/429/