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Identification of arsenic resistant endophytic bacteria from Pteris vittata roots and characterization for arsenic remediation application

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Title Identification of arsenic resistant endophytic bacteria from
Pteris vittata roots and characterization for arsenic remediation application
 
Creator Tiwari, S
Sarangi, B K
Thul, S
 
Subject Environmental Biotechnology
Biochemistry
 
Description Mitigation of arsenic (As) pollution is a topical environmental issue of high R & D priority. The present
investigation was carried out to isolate As resistant endophytes from the roots of Indian ecotype Pteris
vittata and characterize their As transformation and tolerance ability, plant growth promoting characteristics and their role to facilitate As uptake by the plant. A total of 8 root endophytes were isolated from plants grown in As amended soil (25 mg As kg�1). These isolates were studied for minimum inhibitory concentration (MIC), arsenite As(III)-arsenate As(V) transformation ability, plant growth promoting (PGP) characteristics through siderophore, indole acetic acid (IAA) production, phosphatase, ACC deaminase activity, and presence of arsenite oxidase (aox) and arsenite transporter (arsB) genes. On the basis of 16S rDNA sequence analysis, these isolates belong to Proteobacteria, Firmicutes and Bacteroidetes families under the genera Bacillus, Enterobacter, Stenotrophomonas and Rhizobium. All isolates were found As tolerant, of which one isolates showed highest tolerance up to 1000 mg L�1 concentration in SLP
medium. Five isolates were IAA positive with highest IAA production up to 60 mg/L and two isolates exhibited siderophore activity. Phosphatase activity was shown by only one isolate while ACC deaminase activity was absent in all the isolates. The As transformation study by silver nitrate test showed that only two strains had dual characteristics of As(III) oxidation and As (V) reduction, four strains exhibited either of the characteristics while other two didn’t confirmed any of the two characteristics. Presence of aox gene was detected in two strains and arsB
gene in six isolates. The strain with highest As tolerance also showed highest IAA production and occurrence of arsB
gene. Present investigation may open up further scope of utilizing these endophytes for up gradation of phytoextraction process.
 
Publisher Elsevier
 
Date 2016-09-15
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/619/1/Pv-endophytes.pdf
Tiwari, S and Sarangi, B K and Thul, S (2016) Identification of arsenic resistant endophytic bacteria from Pteris vittata roots and characterization for arsenic remediation application. Journal of Environmental Management, 180. pp. 359-365. ISSN 0301-4797, ESSN: 1095-8630
 
Relation http://www.elsevier.com/locate/jenvman
http://neeri.csircentral.net/619/