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Antibiofilm activity of biomolecules: gene expression study of bacterial isolates from brackish and fresh water biofouled membranes

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Title Antibiofilm activity of biomolecules: gene expression study
of bacterial isolates from brackish and fresh water
biofouled membranes
 
Creator Pal, Smita
Qureshi, Asifa
Purohit, H J
 
Subject Environmental Biotechnology
Microbiology
 
Description Membrane biofouling is a common and emerging problem, where cells get cemented and create problems in
industrial process. Frequent chemical cleaning used for the treatment of biofouled membrane shortens the membrane life
time and creates ‘stress’ to existing microflora to trigger more exopolysaccharides production, which becomes the principle
cause of biofouling. To understand safe and environmentally feasible antifouling strategies, key biofilm forming representative
bacteria isolated from brackish and fresh water biofouled membranes were subjected to natural agents, such as vanillin
(0.05–0.4 mg/mL) and salicylic acid (0.1–0.7 mg/mL). Salicylic acid (0.7 mg/mL) was found to be effective against only
Pseudomonas group, whereas vanillin was remarkably potent against majority of the isolates because of structural mimicking
with signalling autoinducer molecules. The present study showed that vanillin served as a good quorum quencher molecule as
it inhibited 90% acyl homoserine lactones production at 0.3 mg/mL concentration in biosensor Chromobacterium violaceum
CV026 strain and also inhibited 70% to > 90% biofilm formation in bacterial isolates. Biofilm formation and quorum sensing
inhibition activities were validated by real-time quantitative PCR gene expression analysis in key representative membrane
isolates. Vanillin served as antifouling natural agent towards broad spectrum community found on membranes.
 
Publisher De Gruyter
 
Date 2016-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/628/1/ASIFA_BIOLOGIA.pdf
Pal, Smita and Qureshi, Asifa and Purohit, H J (2016) Antibiofilm activity of biomolecules: gene expression study of bacterial isolates from brackish and fresh water biofouled membranes. Biologia, 71 (3). pp. 239-246. ISSN (Online) 1336-9563, ISSN (Print) 0006-3088
 
Relation http://www.degruyter.com/journals/biolog
http://neeri.csircentral.net/628/