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Enhanced expression of catechol 1,2 dioxygenase gene in biofilm forming Pseudomonas mendocina EGD-AQ5 under increasing benzoate stress

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Title Enhanced expression of catechol 1,2 dioxygenase gene in biofilm
forming Pseudomonas mendocina EGD-AQ5 under increasing benzoate
stress
 
Creator Ghosh, Saheli
Qureshi, Asifa
Purohit, Hemant
 
Subject Environmental Biotechnology
Microbiology
 
Description In natural settings, microbes often associate closely with surfaces and interfaces called biofilms and
adhere in an extracellular matrix. The biofilm provide diverse microenvironments and high resistance to
environmental stress on the constituent cells that aid to generate cellular heterogeneity and phenotypic
variability. The work presented here examines for the first time the capability of Pseudomonas mendocina
EGD-AQ5 biofilm-associated cells, compared with that of planktonic cells to utilize toxic intermediate
catechol under benzoate degradation conditions in liquid culture systems. Both planktonic and biofilmassociated
cells utilized benzoate to the same extent but accumulated catechol differently. This differential
catechol accumulation activity of the Pseudomonas mendocina EGD-AQ5 could be accredited to the
cells adapted as a biofilm. Further experiments with transcriptome implicated to increased expression of
genes like pelA (involved in biofilm formation) cat1,2D (ortho pathway) and decreased expression of
cat2,3D (meta pathway) in biofilm harvested cells than free suspended cells. Whole genome sequence
analysis of Pseudomonas mendocina EGD-AQ5 (Genbank Accession No AVQF00000000) deciphered
catechol ortho and meta-pathways for benzoate utilization and pelABCDEFG as involved in biofilm formation.
These results suggest that Pseudomonas mendocina EGD-AQ5 biofilms should facilitate bioaugmentation
to be more resilient and efficient bioremediation technology than free suspended
planktonic cells.
 
Publisher Elsevier
 
Date 2017-03
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/700/1/SAHELI_IBB_2017_paper.pdf
Ghosh, Saheli and Qureshi, Asifa and Purohit, Hemant (2017) Enhanced expression of catechol 1,2 dioxygenase gene in biofilm forming Pseudomonas mendocina EGD-AQ5 under increasing benzoate stress. International Biodeterioration & Biodegradation, 118. pp. 57-65. ISSN 0964-8305
 
Relation https://www.journals.elsevier.com/international-biodeterioration-and-biodegradation/
http://neeri.csircentral.net/700/