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Identification and monitoring of nitrification and denitrification genes in Klebsiella pneumoniae EGD-HP19-C for its ability to perform heterotrophic nitrification and aerobic denitrification

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Title Identification and monitoring of nitrification and denitrification
genes in Klebsiella pneumoniae EGD-HP19-C for its ability
to perform heterotrophic nitrification and aerobic denitrification
 
Creator Pal, Rajesh R.
Khardenavis, Anshuman
Purohit, H J
 
Subject Industrial Wastewater Treatment & Disposal
Environmental Biotechnology
Biochemistry
Microbiology
 
Description Microbes capable of performing heterotrophic
nitrification and aerobic denitrification simultaneously
have application in nitrogen level management in effluent
treatment plants. Klebsiella pneumoniae EGD-HP19-
C is a metabolically versatile bacterium capable of
utilising NH3–N, NO2–N and NO3–N as sole sources of
nitrogen. The annotation was done for the genes involved
in N-assimilation and N-dissimilation pathways
from the draft genome sequences of this bacterium
(NCBI GenBank accession no. AUTW02000000.1). The
sequence data also suggested possible existence of plasmid
associated with this bacterium. Multiple gene sequence
alignments of glutamine synthetase (gln), hydroxylamine
reductase (har), nitrite reductase (nir), nitric
oxide reductase (nor), assimilatory nitrate reductase
(nas) and respiratory nitrate reductase (nar) genes from
EGD-HP19-C genome were performed to compare sequence
identities with that of closely related bacterial
species. The metabolic pathways were mapped using
KAAS and 3D structures for representative enzyme
sub-units were also elucidated. The study suggested that
the organism, though it has incomplete nitrification and
denitrification pathways still removes the inorganic nitrogen
content from the system via ammonification reaction.
 
Publisher Springer
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/740/1/Genes%20SND_FIGE.pdf
Pal, Rajesh R. and Khardenavis, Anshuman and Purohit, H J (2015) Identification and monitoring of nitrification and denitrification genes in Klebsiella pneumoniae EGD-HP19-C for its ability to perform heterotrophic nitrification and aerobic denitrification. Functional & Integrative Genomics, 15. pp. 63-76. ISSN 1438-793X (print version) 1438-7948 (electronic version)
 
Relation http://www.springer.com/life+sciences/cell+biology/journal/10142
http://neeri.csircentral.net/740/