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Metagenomic approach to characterize soil microbial diversity of Phumdi at Loktak Lake

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Title Metagenomic approach to characterize soil microbial
diversity of Phumdi at Loktak Lake
 
Creator Puranik, Sampada
Pal, Rajesh
More , Ravi
Purohit, H J
 
Subject Water Resources
Microbiology
 
Description Loktak, one of the largest freshwater lakes of India, is known for floating islands (Phumdi), being made
up of a heterogeneous biomass of vegetation and soil. This ecological site represents an exclusive
environmental habitat wherein the rhizospheric microbial community of Phumdi plays a key role in
biogeochemical cycling of nutrients. A culture-independent whole genome shotgun sequencing
based metagenomic approach was employed to unravel the composition of the microbial community
and its corresponding functional potential at this environmental habitat. Proteobacteria (51%) was
found to be the most dominant bacterial phylum followed by Acidobacteria (10%), Actinobacteria (9%)
and Bacteroidetes (7%). Furthermore, Loktak metagenome data were compared with available
metagenomes from four other aquatic habitats, varying from pristine to highly polluted eutrophic
habitats. The comparative metagenomics approach aided by statistical analysis revealed that
Candidatus Solibacter, Bradyrhizobium, Candidatus Koribacter, Pedosphaera, Methylobacterium,
Anaeromyxobacter, Sorangium, Opitutus and Acidobacterium genera are selectively dominant at this
habitat. Correspondingly, 12 different functional categories were found to be exclusively prevalent at
Phumdi compared to other freshwater habitats. These differential features have been attributed to the
unique habitat at Phumdi and correlated to the phenomenon of bioremediation at Loktak Lake
 
Publisher IWA Publishing
 
Date 2016-11
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/812/1/puranik2016_NN.pdf
Puranik, Sampada and Pal, Rajesh and More , Ravi and Purohit, H J (2016) Metagenomic approach to characterize soil microbial diversity of Phumdi at Loktak Lake. Water Science & Technology, 74 (9). pp. 2075-2086. ISSN 0273-1223, ESSN: 1996-9732
 
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