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Evaluation of host Hsp(s) as potential biomarkers for the diagnosisof tuberculous meningitis

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Title Evaluation of host Hsp(s) as potential biomarkers for the diagnosisof tuberculous meningitis
 
Creator Shekhawata, Seema D
Purohit, H J
Taoria, Girdhar M
Daginawala, Hatim F
Kashyap, Rajpal S
 
Subject Environmental Impact
 
Description Objectives: Diagnosis of tuberculosis meningitis (TBM) remains challenging in tuberculosis (TB) endemic
countries. The need for TB biomarkers arises, in part, from the difficulty of accurately diagnosing TBM
with the available methods.
Patients and methods: To explore the potential of host Hsps (Hsp 25, Hsp 60, Hsp 70 and Hsp 90) as
an alternative marker in TBM diagnosis, we evaluated cerebrospinal fluid (CSF) sample of TBM (n = 49),
Pyogenic Meningitis (PM) (n = 20), Viral Meningitis (VM) (n = 09), Fungal Meningitis (FM) (n = 04) and non
infectious control (n = 79) patients using indirect ELISA.
Results: Out of four Hsps, Hsp 70 and Hsp 90 yields 89% & 88% sensitivity and 82% & 89% specificity,
respectively. The positive (PPV) and negative (NPV) predictive values yielded in TBM group for Hsp 70
was 86.27% (73.74–94.27) and 93.51% (85.48–97.83), respectively. For Hsp 90 the obtained PPV was
89.36% (76.88–96.41) and NPV was 91.36% (82.99–96.44). In 86% of TBM patients all the four Hsps were
found to be positive and none of the patient was found to be negative for all Hsps in the same group.
Conclusions: The data presented in the study indicate that host Hsp 70 and Hsp 90 shows good sensitivity
and specificity and have potential in the diagnosis of TBM disease. The combined use of all Hsps (Hsp 25,
Hsp 60, Hsp 70 and Hsp 90) effectively distinguishes patients with TBM from other disease controls.
 
Publisher Elsevier
 
Date 2016
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/813/1/15%20shekhawat2016_N.pdf
Shekhawata, Seema D and Purohit, H J and Taoria, Girdhar M and Daginawala, Hatim F and Kashyap, Rajpal S (2016) Evaluation of host Hsp(s) as potential biomarkers for the diagnosisof tuberculous meningitis. Clinical Neurology and Neurosurgery, 140. pp. 47-51. ISSN 0303-8467, ESSN: 1872-6968
 
Relation http://www.sciencedirect.com/science/journal/03038467/140/supp/C
http://neeri.csircentral.net/813/