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Nested PCR Assay for Eight Pathogens: A Rapid Tool for Diagnosis of Bacterial Meningitis

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Title Nested PCR Assay for Eight Pathogens: A Rapid Tool
for Diagnosis of Bacterial Meningitis
 
Creator Bhagchandani, Sharda P
Kubade, Sushant
Nikhare, Priyanka P
Manke, Sonali
Chandak, Nitin H
Kabra, Dinesh
Baheti, Neeraj N
Agrawa, Vijay S
Sarda, Pankaj
Mahajan, Parikshit
Ganjre, Ashish
Purohit, H J
Singh, Lokendra
Taori, Girdhar M
Daginawala, Hatim F
Kashyap, Rajpal S
 
Subject Environmental Health
 
Description Purpose Bacterial meningitis is a dreadful infectious disease
with a high mortality and morbidity if remained undiagnosed.
Traditional diagnostic methods for bacterial
meningitis pose a challenge in accurate identification of
pathogen, making prognosis difficult. The present study is
therefore aimed to design and evaluate a specific and sensitive
nested 16S rDNA genus-based polymerase chain reaction
(PCR) assay using clinical cerebrospinal fluid (CSF) for
rapid diagnosis of eight pathogens causing the disease.
Methods The present work was dedicated to development
of an in-house genus specific 16S rDNA nested PCR
covering pathogens of eight genera responsible for causing
bacterial meningitis using newly designed as well as literature
based primers for respective genus. A total 150
suspected meningitis CSF obtained from the patients
admitted to Central India Institute of Medical Sciences
(CIIMS), India during the period from August 2011 to May
2014, were used to evaluate clinical sensitivity and clinical
specificity of optimized PCR assays.
Results The analytical sensitivity and specificity of our
newly designed genus-specific 16S rDNA PCR were found
to be C92 %. With such a high sensitivity and specificity,
our in-house nested PCR was able to give 100 % sensitivity
in clinically confirmed positive cases and 100 % specificity
in clinically confirmed negative cases indicating its applicability
in clinical diagnosis.
 
Publisher Springer
 
Date 2016-02
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/814/1/bhadchandani%202016_N.pdf
Bhagchandani, Sharda P and Kubade, Sushant and Nikhare, Priyanka P and Manke, Sonali and Chandak, Nitin H and Kabra, Dinesh and Baheti, Neeraj N and Agrawa, Vijay S and Sarda, Pankaj and Mahajan, Parikshit and Ganjre, Ashish and Purohit, H J and Singh, Lokendra and Taori, Girdhar M and Daginawala, Hatim F and Kashyap, Rajpal S (2016) Nested PCR Assay for Eight Pathogens: A Rapid Tool for Diagnosis of Bacterial Meningitis. Molecular Diagnosis and Therapy, 20 (1). pp. 45-54. ISSN 1177-1062, ESSN: 1179-2000
 
Relation https://link.springer.com/journal/40291
http://neeri.csircentral.net/814/