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Genomic and functional features of the biosurfactant producing Bacillus sp. AM13

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Title Genomic and functional features of the biosurfactant producing Bacillus sp. AM13
 
Creator Shaligram, Shraddha
Kumbhare, Shreyas V
Dhotre, Dhiraj P
Muddeshwar, Manohar G
Kapley, Atya
Joseph, Neetha
Purohit, H P
Shouche, Yogesh S
Pawar, Shrikant P
 
Subject Microbiology
 
Description Genomic studies provide deeper insights into
secondary metabolites produced by diverse bacterial communities,
residing in various environmental niches. This study
aims to understand the potential of a biosurfactant producing
Bacillussp. AM13, isolated from soil. An integrated approach
of genomic and chemical analysis was employed to characterize
the antibacterial lipopeptide produced by the strain AM13.
Genome analysis revealed that strain AM13 harbors a
nonribosomal peptide synthetase (NRPS) cluster; highly
similar with known biosynthetic gene clusters from surfactin
family: lichenysin (85 %) and surfactin (78 %). These findings
were substantiated with supplementary experiments of oil
displacement assay and surface tension measurements,
confirming the biosurfactant production. Further investigation
using LCMS approach exhibited similarity of the biomolecule
with biosurfactants of the surfactin family. Our consolidated
effort of functional genomics provided chemical as well as
genetic leads for understanding the biochemical characteristics
of the bioactive compound.
 
Publisher Springer
 
Date 2016-09
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/815/1/shaligram%202016_N.pdf
Shaligram, Shraddha and Kumbhare, Shreyas V and Dhotre, Dhiraj P and Muddeshwar, Manohar G and Kapley, Atya and Joseph, Neetha and Purohit, H P and Shouche, Yogesh S and Pawar, Shrikant P (2016) Genomic and functional features of the biosurfactant producing Bacillus sp. AM13. Functional & Integrative Genomics, 16 (5). pp. 557-566. ISSN 1438-793X, ESSN: 1438-7948
 
Relation https://link.springer.com/journal/10142
http://neeri.csircentral.net/815/