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Evaluation of heat shock proteins fordiscriminating between latenttuberculosis infection and activetuberculosis: A preliminary report

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Title Evaluation of heat shock proteins fordiscriminating between latenttuberculosis infection and activetuberculosis: A preliminary report
 
Creator Shekhawata, Seema D
Purohit, H J
Taoria, Girdhar M
Daginawala, Hatim F
Kashyap, Rajpal S
 
Subject Environmental Health
 
Description The diagnosis of a latent tuberculosis infection (LTBI) is of the utmost
concern. The available tests, the tuberculin skin test (TST) and the QuantiferonTB
Gold test (QFT-G) cannot discriminate between active TB and LTBI. Therefore,
the aim of the study is to identify new biomarkers that can discriminate between
active TB and LTBI and can also assess the risk of the individual developing active
TB. In total, 55 blood samples were collected, of which 10 samples were from the
active TB infection group, 10 were from the high-risk exposure group, 23 were from
the low-risk exposure group, and 12 were from healthy controls living in a non-TB
endemic area. A panel of heat shock proteins (Hsps), including host Hsp25, Hsp60,
Hsp70, and Hsp90 and Mycobacterium tuberculosis (MTB) Hsp16, were evaluated
in all of the collected samples using ELISA. The levels of the host Hsp(s) (Hsp25,
Hsp60, Hsp70 and Hsp90) and MTB Hsp16 were significantly (p < 0.05) elevated in the
active TB group compared to the high-risk exposure group, the low-risk exposure
group and the control group. Notably, the levels of the same panel of Hsp(s) were
elevated in the high-risk exposure group compared to the low-risk exposure group.
On follow-up, out of the 10 high-risk exposure participants, 3 converted into active TB, indicating that this group has the highest risk of developing TB. Thus, the evaluated
panel of Hsp(s) can discriminate between LTBI and active TB. They can also
identify individuals who are at the highest risk of developing active TB. Because they
can be rapidly detected, Hsp(s) have an edge over the existing diagnostic tools for
LTBI. The evaluation of these proteins will be useful in designing better diagnostic
methods for LTBI.
 
Publisher Elsevier
 
Date 2016-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://neeri.csircentral.net/816/1/Shekhawat_NN.pdf
Shekhawata, Seema D and Purohit, H J and Taoria, Girdhar M and Daginawala, Hatim F and Kashyap, Rajpal S (2016) Evaluation of heat shock proteins fordiscriminating between latenttuberculosis infection and activetuberculosis: A preliminary report. Journal of Infection and Public Health, 9 (2). pp. 143-152. ISSN 1876-0341, ESSN: 1876-035X
 
Relation https://www.journals.elsevier.com/journal-of-infection-and-public-health/
http://neeri.csircentral.net/816/